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Assisted Embryo Hatching (AEH) is another form of micromanipulation used at the ART Program of Alabama. Unlike ICSI, AEH focuses on the embryo rather than the egg. AEH is helpful in cases where there may be an expected problem for the embryo to break out of its shell, the zona pellucida. This includes patients with failed cycles, abnormal hormone levels or suboptimal embryo quality. A microneedle filled with a chemical is used to introduce a small opening in the zona pellucida. Introducing this opening can facilitate the embryo's release from the zona pellucida. This may result in increased implantation in the uterus.

There can be no guarantee, however, that all eggs fertilized will produce an embryo, with or without the use of micromanipulation. IVF technology is not yet capable of determining which eggs will fertilize, or which ones will produce the most viable embryos.

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